IL-8在HBV相关慢加急性肝衰竭中的临床意义

Clinical significance in detection of serum levels of IL-8 in patients with HBV-ACLF

目的探讨白细胞介素(IL)-8在乙型肝炎相关慢加急性肝功能衰竭(HBV-ACLF)中的临床价值。方法对62例HBV-ACLF患者和57例HBV患者采用免疫组化技术分析肝组织IL-8水平与分布情况,利用ELISA技术测定血清IL-8水平。对其HBV-DNA和血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素和肌酐等肝功能指标水平亦进行同步测定。结果HBV-ACLF患者肝组织及血清中IL-8含量均明显高于HBV患者及健康对照者;且经Pearson相关性分析,HBV>500 IU/ml时血清IL-8与HBV-DNA水平呈正相关性(r=0.416,P<0.05)。与HBV患者和健康对照者相比,HBVACLF患者血清中ALT、AST、ALP、总胆红素和肌酐含量均有明显增加。血清IL-8与肝损害程度(ALT、AST水平)亦呈显著正相关性(r=0.247、0.317,P<0.05)。结论 HBV-ACLF患者肝组织及外周血IL-8水平显著升高,且血清IL-8水平与患者肝脏炎症损伤和病情严重程度有关。

Objective To investigate the clinical significance of serum levels of IL-8 in patients with HBV-ACLF.Methods The level of interlukin-8（IL-8） in liver tissue were analysed by immunohistochemistry in totally 62HBV-ACLF patients and 57 patients with chronic hepatitis B（CHB）. ELISA was used to detect the serum level of IL-8. Meanwhile,HBV DNA and several liver function indexes such as alanine aminotransferase（ALT）,aspartate transaminase（AST）,alkaline phosphatase（ALP）,total bilirubin（TBIL） and creatinine（Cr） were examined. Results IL-8 levels in serum and liver tissues of HBV-ACLF patients were both significantly higher than those in HBV group and healthy control group（P〈0. 05）. Through the Pearson correlation analysis,serum IL-8 quantification was positively correlated with HBV-DNA load when HBV 500 IU/ml（r = 0. 416,P〈0. 05）. Compared with HBV patients and healthy controls,liver function indicators such as ALT,AST,ALP,TBIL and Cr were obviously increased in HBV-ACLF patients. In addition,serum IL-8 quantifications were positively correlated with ALT and AST（r = 0. 247,0. 317,P〈0. 05）. Conclusion IL-8 level in serum and liver tissue of HBV-ACLF patients increase significantly,and the serum IL-8 level is related to the degree of liver inflammation injury and the severity of HBV-ACLF.