采用高速离心及ExoQuick-TC法提取小鼠骨髓间充质干细胞来源外泌体比较及鉴定

Comparison of ultracentrifugation and ExoQuick-TC for the identification of exosomes extracted from mouse bone marrow mesenchymal stem cells

比较及鉴定超高速离心法及ExoQuick-TC法提取小鼠骨髓间充质干细胞来源外泌体。采用BCA蛋白检测可见ExoQuick-TC法提取的外泌体蛋白含量高于超高速离心法提取的蛋白含量。Exosome Antibodies,Array&ELISA Kits检测可见ExoQuick-TC法提取外泌体的纯度优于超高速离心法提取的纯度。两种方法提取的外泌体电镜下形状无差异,随机选取3个电镜视野进行计数可见ExoQuick-TC法提取外泌体数量多于超高速离心法提取外泌体。ExoQuick-TC法提取的外泌体具有更高的产量及纯度。

To compare ultracentrifugation and ExoQuick-TC in identifying the characteristics of exosomes extracted from mouse bone marrow mesenchymal stem cells. BCA protein assay revealed that the protein contents in the ExoQuick-TC-extracted exosomes were higher than those in the ultracentrifugation-extracted exosomes. Analysis using exosome antibodies,array,and ELISA kits indicated that the purity of the ExoQuick-TC-extracted exosomes was higher that of the ultracentrifugation-extracted exosomes. Electronmicroscopy demonstrated that the morphological characteristics of the ExoQuick-TC-extracted exosomes did not significantly differ from those of the ultracentrifugation-extracted exosomes. Three electron microscope visual fields were randomly selected to count the number of exosomes. Results showed that the number of the ExoQuick-TC-extracted exosomes was more than that of the ultracentrifugation-extracted exosomes. Therefore,the production and purity of ExoQuick-TC-extracted exosomes are higher than those of ultracentrifugation-extracted exosomes.