摘要
The electrospun nanofibrous scaffolds made of proteins and polysaccharides were thought to be able to simulate the structure of natural extracellular matrix well.Silk fibroin(SF)and chitosan(CS)are probably the most widely used natural materials in biomedical fields including liver tissue engineering for their good properties and wide variety of sources.The asialoglycoprotein receptors of hepatocyte were reported to specifically recognize and interact with galactose.In this work,a green electrospun SF/galactosylated chitosan(GC)composite nanofibrous scaffold was fabricated and characterized.The data indicated that the addition of GC greatly influenced the spinning effect of SF aqueous solution,and the average diameter of the composite nanofibers was about 520nm.Moreover,the green electrospun SF/GC nanofibrous scaffolds were demonstrated significantly enhancing the adhesion and proliferation of hepatocyte(RH35)according to our data.The present study did a useful exploration on constructing scaffolds for liver regeneration by green electrospinning,and also laid a good foundation for the further applicative research of this green electrospun scaffolds in liver tissue engineering.
The electrospun nanofibrous scaffolds made of proteins and polysaccharides were thought to be able to simulate the structure of natural extraceliular matrix well. Silk fibroin （SF） and chitosan （CS） are probably the most widely used natural materials in biomedical fields including liver tissue engineering for their good properties and wide variety of sources. The asialoglycoprotein receptors of hepatocyte were reported to specifically recognize and interact with galactose. In this work, a green eleetrospun SF/ galactosylated chitosan （GC） composite nanofibrons scaffold was fabricated and characterized. The data indicated that the add/tion of GC greatly influenced the spinning effect of SF aqueous solution, and the average diameter of the composite nanofibers was about 520 nm. Moreover, the green electrospun SF/GC nanofibrous scaffolds were demonstrated significantly enhancing the adhesion and proliferation of bepatocyte （ RH35 ） according to our data. The present study did a useful exploration on constructing scaffolds for liver regeneration by green electrospinning, and also laid a good foundation for the further applicative research of this green eleetrospan scaffolds in liver tissue engineering.
基金
“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)
Natural Science Foundation of Shanghai,China(No.12ZR1400300)
the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry
