Fabrication and Characterization of Dual-layer Multichannel Nerve Guidance Conduit

Nowadays,multichannel nerve guidance conduit(NGC)was designed by mimicking the architecture of nerve fascicles,and it was used to reduce dispersion of regenerating axons within the NGC lumen.In this paper,gelatin was used to prepare multichannel inner layer of NGC by freeze-drying,and poly(L-lactic acid-co-ε-caprolactone)(P(LLA-CL))was used to fabricate nanofiber outer layer of NGC by electrospinning.The morphology of dual-layer multichannel NGC was observed by scanning electron microscopy(SEM).In vitro degradation experiment of the NGC demonstrated that the inner layer of NGC had the faster degradation rate than the outer layer of NGC.cell viability assay indicated that Schwann cells(SCs)showed better proliferation on dual-layer multichannel NGC than hollow NGC,because the multichannel structure introduced contact guidance for direct cell migration.Therefore,it was suggested that the dual-layer multichannel NGC had the potential for nerve tissue regeneration.

Nowadays, muifichannel nerve guidance conduit （NGC） was designed by mimicking the architecture of nerve fascicles, and it was used to reduce dispersion of regenerating axons within the NGC lumen. In this paper, gelatin was used to prepare multichannel inner layer of NGC by freeze-drying, and poly （ L-lactic add-co-ε- caprolactone） （P（LLA-CL）） was used to fabricate nanofiber outer layer of NGC by electrospinning. The morphology of dual-layer mtlltichannel NGC was observed by scanning electron microscopy （SEM）. In vitro degradation experiment of the NGC demonstrated that the inner layer of NGC had the faster degradation rate than the outer layer of NGC. tell viability assay indicated that Schwann cells （SCs） showed better proliferation on dual-layer multichannel NGC than hollow NGC, because the multichannel structure introduced contact guidance for direct cell migration. Therefore, it was suggested that the dual-layer multichannel NGC had the potential for nerve lissue regeneration.