消喘膏的体外透皮吸收研究

In vitro Transdermal Absorption of Xiaochuan Ointment

目的:研究消喘膏中芥子碱硫氰酸盐、延胡索乙素和细辛脂素成分的体外经皮渗透性,为其临床应用提供参考。方法:采用Franz扩散池法,用LC-MS/MS法测定膏中芥子碱硫氰酸盐、延胡索乙素和细辛脂素成分在一段时间内对大鼠离体皮肤的透皮吸收量。结果:不同给药量对各成分的累积渗透量影响不大。模型拟合结果显示芥子碱硫氰酸盐透皮行为符合Higuchi方程,延胡索乙素和细辛脂素符合零级方程,延胡索乙素和细辛脂素的透皮速率分别为0.362×10^(-1),0.330×10^(-2)μg·cm^(-2)·h^(-1)。结论:消喘膏具有经皮渗透性能,为其透皮吸收给药途径提供了依据。

Objective： To study the transdermal absorption in vitro of sinapine thiocyanate, tetrahydropalmatine and asarinin in Xiaochuan ointment so as to offer reference for its clinical application. Methods： A Franz diffusion cells method with isolated rat skins was carried out to study the pereutaneous rate of sinapine thioeyanate, tetrahydropalmatine and asarinin determined by LC-MS/MS. Results： With the increase of administration dosage, the cumulative penetration of sinapine thioeyanate,tetrahydropalmatine and asarinin showed few changes. The results showed that the transdermal behavior of sinapine thioeyanate fit to a Higuchi equation, and that of tetrahydropalmatine and asarinin fit zero-order process. The penetration rate of tetrahydropalmatine and asarinin respectively was 0.362×10^-1,0.330×10^-2μg·cm^-2·h^-1. Conclusion： Xiaoehuan ointment exhibits transdermal penetration and absorption properties, which provides evidence for its transdermal administration.