循环肿瘤细胞检测对直肠癌同步放化疗的临床意义

Clinical significance of circulating tumor cells for chemo-radiotherapy in patients with rectal cancer

目的探讨外周血循环肿瘤细胞(CTCs)对直肠癌患者同步放化疗的临床意义。方法选取2014年6月至2015年12月间新疆医科大学第五附属医院收治的行同步放化疗的30例直肠癌患者为观察组,同时随机选取20例健康体检者为对照组。采用密度离心法及免疫荧光技术的方法,进行外周血循环肿瘤细胞检测,同时分析同步放化疗前后CTCs与患者临床特征的变化情况。结果直肠癌患者外周血中检出CTCs者18例,阳性检出率为76.2%;对照组患者中未检出CTCs,且CTCs阳性检出率与患者临床分期和淋巴结转移情况有关,差异均有统计学意义(均P<0.05)。结论CTCs与直肠癌患者的不良预后相关,提示直肠癌患者有较高的恶性风险及潜在转移风险。

Objective To explore the clinical significance of circulating tumor cells （CTCs） in the peripheral blood for patients with rectal cancer receiving ehemo-radiotherapy （CRT）. Methods Thirty patients with rectal cancer who had received CRT in The Fifth Affiliated Hospital of Xinjiang Medical University from June 2014 to December 2015 were selected as the observation group and 20 healthy volunteers were selected as the control group. CTCs were detected using density-gradient eentrifugation and immunofluores- cence staining. The changes in CTCs level and clinical characteristics were also analyzed pre- and post- chemoradiotherapy. Results CTCs were detected in 18 patients, and the positive rate of CTCs in the peripheral blood for rectal cancer group was 76. 2% , while no tumor cell could be found in the control group. The positive rate of CTCs was significantly correlated with clinical stages and lymph node metastasis （ all P 〈 O. 05 ）. Conclusion CTCs was associated with poor prognosis of rectal cancer, which predicted malignant eoloreetal risk and potential risk of metastasis.