大黄鱼鱼卵磷脂酰胆碱的分离纯化

Isolation and purification of phosphatidylcholine from large yellow croaker roe

以氯仿/甲醇法提取的大黄鱼鱼卵粗磷脂为原料,研究柱层析固定相种类、粒度,洗脱剂流速、配比及样品上载量等因素对分离纯化的鱼卵磷脂酰胆碱(Roe-PC)纯度和回收率的影响,以确定最佳的工艺参数.结果表明,在固定相为200~300目硅胶,流动相为氯仿/甲醇(3∶2),流速为2 m L·min^(-1),上载量为0.035 g·g^(-1)(样品/硅胶)的条件下,分离纯化的大黄鱼Roe-PC的纯度可达96.34%,回收率为85.18%.

Phosphatidylcholine（ PC） was purified from phospholipids of large yellow croaker（ Pseudosciaena crocea） roe by chloroform/methanol system. Parameters including types of stationary phase,granularity,eluent rate and composition,loading quantity were investigated to optimize its yield and purity. The results showed that the optimum procedure was to co-eluate sample from silica gel 200-300 using chloroform/methanol（ 3 ∶ 2） at the rate of 2 m L·min^-1,with loading amount being 0.035 g·g^-1（ sample/silica gel）. Under this condition,the purity of PC can reach 96.34%,with recovery rate being 85.18%.