吗啡诱导的大鼠条件性位置偏爱模型中关键脑区PEBP及ERK活性的变化

Analysis of brain PEBP and ERK in morphine-induced CPP model in rats

目的研究大鼠阿片精神依赖状态下,相关脑区磷脂酰乙醇胺结合蛋白(PEBP)及ERK活性的变化。方法建立条件性位置偏爱(CPP)模型模拟吗啡精神依赖的不同阶段,观察依赖相关重要脑区在大鼠CPP形成、熄灭及重建时PEBP表达和ERK活性的变化。结果海马、前额叶皮层、纹状体、伏隔核等4个脑区,CPP各阶段均未见PEBP表达水平有明显变化;但在CPP形成时,前额叶皮层的ERK活性明显升高,而CPP重建后,海马的ERK活性明显降低。结论吗啡成瘾不同阶段,药效-环境关联性记忆的形成和唤起涉及不同神经通路,ERK活性在其中十分关键,但PEBP可能并未参与ERK活性调节。

Aim To analyze the expression of phosphatidylethanolamine-binding protein（ PEBP） and ERK in critical brain regions of psychological dependence rats.Methods Morphine-induced rats conditioned place preference（ CPP） model was established to mimic different stages of morphine psychological dependence,during which PEBP expression and ERK activity were assayed in different brain regions.Results PEBP expression in hippocampus,prefrontal cortex,striatum and nucleus accumbens showed no change at three stages of psychological dependence.However,ERK activity increased notably in prefrontal cortex on CPP formation,and decreased remarkably in hippocampus on CPP reinstatement.Conclusions The formation and retrieval of associated memory between morphine effects and environment involve different neural circuits,in which ERK activity is critical,and PEBP might not be involved in such a memory-related ERK regulation.