谷胱甘肽高产酵母融合子的制备及发酵工艺

Preparation and fermentation process of GSH high-yield yeast fusant

为构建谷胱甘肽(glutathione,GSH)高产酵母菌株,本实验利用原生质体融合方法制备多形汉逊酵母和粟酒裂殖酵母融合子,结合自主设计的耐高温、钼酸钠和乙醇耐受性培养基方法高通量筛选酵母融合子,进而利用DTNB法定量测定GSH高产酵母融合子,并分析GSH高产酵母融合子的遗传稳定性,最后对其发酵工艺进行研究.结果表明,本实验利用原生质融合方法成功构建一株GSH产量高、遗传稳定的GSH高产酵母融合子,为低成本、高效生产GSH提供了新途径.

To construct glutathione（GSH） high-yield yeast fusant,the fusants were derived from protoplast fusion between Hansenula polymorpha and Schizosaccharomyce spombe by polyethylene glycol（PEG） fusion method.Based on-high temperature screening was adopted in prescreening,and ethanol and Sodium molybdate culture medium were utilized in second screening.Then GSH content was determined by 5,5＇-Dithio bis-（2-nitrobenzoic acid）（DTNB）.Finally,the genetic stability of the glutathione（GSH） high-yield yeast fusants was analyzed.One GSH yeast fusant strain high-yield and genetic stability was constructed.Therefore,a breeding strategy based on the protoplast fusion method would be a valuable alternative to improve glutathione production.