活血消异方对子宫内膜异位症模型小鼠子宫内膜容受性的影响研究

Effects of Huoxuexiaoyi recipe on endometrial receptivity in mice models with endometriosis

目的探讨子宫内膜异位症对子宫内膜容受性的影响机制以及活血消异方对子宫内膜异位症模型小鼠子宫内膜容受性的作用。方法通过"皮下+腹腔"同系异体子宫内膜注射的方法建立子宫内膜异位症妊娠功能低下小鼠模型,假手术予等量0.9%氯化钠溶液注射。建模成功后,随机分为中药组和模型组,中药组予活血消异方干预治疗,模型组和假手术组予等量0.5%羧甲基纤维素钠(CMC)灌胃。连续治疗3个周期后,3组分别与不育雄鼠合笼,次日清晨8∶00观察阴栓,见栓者定为假孕第1天,于假孕第4天晚上10∶30取材,运用Western blot法检测小鼠子宫内膜整合素αvβ3和LIF的蛋白表达。结果模型组小鼠种植窗口期子宫内膜整合素αvβ3和LIF的蛋白表达量较假手术组显著降低,差异有统计学意义(P<0.05);中药组小鼠种植窗口期子宫内膜整合素αvβ3和LIF的蛋白表达量较模型组显著升高,差异有统计学意义(P<0.05),但均低于假手术组(P<0.05)。结论子宫内膜异位症对子宫内膜容受性存在不良影响。活血消异方能够显著提高小鼠围着床期子宫内膜着床因子整合素αvβ3和LIF的蛋白表达量,从而改善子宫内膜异位症模型小鼠子宫内膜容受性。

Objective To investigate the effects of Huoxuexiaoyi recipe on endometrial receptivity in mice models with endometriosis,and to explore its action mechanism.Methods The animal models with endometriosis pregnancy hypofunction were established in mice through the method of abdominal cavity ＋ subcutaneous injections,and the mice in sham-operation group were injected with same volume of 0.9% sodium chloride solution.After the success of modeling,the mice were randomly divided into traditional Chinese medicine group（TCM group） and model group.The mice in TCM group were treated by Huoxuexiaoyi recipe,however,the mice in model group and sham-operation group were given same volume of0.5% CMC by gavage.After three menstrual cycles,the mice in the three groups were put in the cage with infertile male mice（2∶ 1）,then the vaginal plug was observed at 8∶ 00 am in the next day（D1）.The detection specimens were taken at 10∶ 30 pm on D4 after false pregnant,and the expression levels of endometrial integrin-αvβ3 and leukaemia inhibitory factor（LIF）were detected by Western Blot.Results The expression levels of endometrial integrin-αvβ3 and LIF at implantation window phase in model group were significantly decreased,as compared with those in sham-operation group（P〈0.05）.However the expression levels of endometrial integrin-αvβ3 and LIF at implantation window phase in TCM group were significantly increased,as compared with those in model group（P〈0.05）,but which were significantly lower than those in sham-operation group（P〈0.05）.Conclusion The endometriosis has adverse effects on endometrial receptivity.Huoxuexiaoyi recipe can significantly increase the expression levels of endometrial integrin αvβ3 and LIF at implantation window phase so as to improve endometrial receptivity in mice with endometriosis.