亚麻AtCesA1基因过表达载体的构建

Cellulose Synthase Gene AtCesA1's Expression Vector Construction of Transforming Flax

为开展纤维素合成酶基因导入亚麻的基因工程研究,以拟南芥为材料克隆了纤维素合成酶基因AtCesA1,序列长为3912 bp,其中编码区在277~3523碱基之间,共3246 bp,推测其编码了1082个氨基酸。通过同义突变将全长基因克隆到植物表达载体pBI1301中,构建出植物表达载体pCAMBIA1301-AtCesA1,经酶切和PCR鉴定确认目的基因已经正确地插入到载体上,为下一步AtCesA1遗传转化亚麻功能研究打下了基础。

In order to introduce cellulose synthase gene into flax by agrobacterium mediated method, the cellulose synthase gene AtCesAl was cloned from Arabidopsis thaliana. Sequence analysis showed that the coding region was located between the 277 -3523 base,a total of 3246 bp,which presumably encoded 1082 amino acids, and the total length of cellulose synthase gene was 3912 bp. The full length AtCesAl cDNA was subcloned into pBI1301 by synonymy mutation, constructing a higher plant expression vector pCAMBIA1301 - AtCesAl. The construction of the vector was verified by enzyme digestion and PCR iden-tification, which can lay a foundation for further research on the function of AtCesAl gene transformation.