复方柴金解郁片对慢性应激抑郁大鼠HPA轴及海马单胺神经递质的影响

Effects of Compound Chaijin Jieyu Tablets on HPA Axis and Hippocampal Monoamine Neurotransmitter in Chronic Stress Depression Rats

目的研究复方柴金解郁片对慢性应激抑郁模型大鼠HPA轴及海马单胺神经递质的影响,探讨其可能的抗抑郁机制。方法将SD大鼠随机分为6组:对照组、模型组、氟西汀组(5.4 mg/kg)、复方柴金解郁片高、中、低剂量组(2.74、1.37、0.68 g/kg),采用慢性不可预见性温和应激建立抑郁模型,于造模同时给药,连续28 d。采用蔗糖偏好实验和旷场测试检测大鼠的抑郁样行为,Morris水迷宫检测大鼠的学习记忆能力,HE染色观察大鼠海马病理结果变化,ELISA法检测大鼠血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT),以及海马5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、去甲肾上腺素(NA)、多巴胺(DA)的含量。结果与抑郁模型组比较,复方柴金解郁片组大鼠蔗糖偏嗜度及活动次数均显著提高,学习记忆能力提升,海马病理损伤情况得到缓解,血浆CRH、ACTH、CORT含量下降,海马组织内5-HT、NA、DA含量上升,5-HIAA含量下降(P<0.05)。结论复方柴金解郁片能显著改善模型大鼠情绪和学习记忆功能、缓解海马组织损伤,其作用可能与抑制HPA轴亢进、增加脑内单胺递质含量有关。

Objective To study the effect of compound Chaijin Jieyu tablets on HPA axis and hippocampal monoamine neurotransmitter in chronic unpredictable stress model rats, and to explore its possible antidepressant mechanism. Methods SD rats were randomly divided into six groups： the control group, model group, fluoxetine group（5.4 mg/kg）,and high, medium and low dose of compound Chaijin Jieyu tablets group（2.74,1.37,0.68 g/kg）. Chronic unpredictable mild stress was used to build the model of depression, and the model was administered simultaneously for 28 days. Sugar preference test and open field test were used to test the depressive behavior of rats, Morris water maze was used to test the ability of learning and memory of the rats. Pathological changes of hippocampus were observed by HE staining. Serum CRH, ACTH,CORT, hippocampal 5-HT, 5-HIAA, NA and DA levels were measured by ELISA. Results Compared with model group,the degree of sucrosepreference, activities, learning and memory ability were significantly increased. Hippocampal pathological damage were alleviated. Levels of CRH, ACTH and CORT in plasma decreased, and 5-HT, NA and DA in hippocampus increased, the content of 5-HIAA decreased. Conclusion Compound Chaijin Jieyu tablets could significantly improve the mood,learning and memory function of rats, alleviate the injury of hippocampus. The role of Chaijin Jieyu tablets might be related to inhibition of HPA axis hyperactivity, and increasing the content of monoamine transmitters in the brain.