黄连解毒汤对大鼠营养性肥胖的影响

Effects of Different Doses of Huanglian Jiedu Tang on Rats with Nutritional Obesity

目的:观察黄连解毒汤低、中、高剂量组给药时间长短对营养性肥胖大鼠的影响。方法:100只SD雄性大鼠按体重随机分为正常组、模型组、黄连解毒汤组(高、中、低剂量组),每组20只,除正常组外,其余各组用"高脂乳剂+碳酸饮料+普通饲料"混合喂养造大鼠营养性肥胖模型,连续3周,3周后分别于给药22d、42d,每组各处理10只大鼠,分离血清,剥离肾及生殖器周围脂肪组织,检测血清GLU、TG、TC、HDL-C、LDL-C和脂肪组织CEBPα含量,计算脂肪指数。结果:与模型组比较,各给药组可明显降低肥胖大鼠体重、脂肪指数,明显升高肥胖大鼠HDL-C水平,其中黄连解毒汤中剂量组能明显降低TG、TC、LDL-C、CEBPα水平。以上结果均具有统计学意义(P<0.05或P<0.01)。结论:黄连解毒汤对大鼠营养性肥胖具有一定的干预作用,其中中剂量组作用尤为突出,除HDL-C,对其它指标的影响给药时间均不宜过长。

Objective： To observe the effects of Huanglian Jiedu Tang（ HLJDT） with different dosage and administration time on indexes of rats with nutritional obesity. Methods： 100 male SD rats were randomly divided into normal group,model group,low dose group,medium dose group and high dose group,20 in each group. In addition to the normal group,the rest of the groups were made a model of rats with nutritional obesity by using the ＂ high fat emulsion ＋ sodas ＋ ordinary diet＂ way for 3 weeks. The administration time after model establishment were 22 days and 42 days. 10 rats were to be sacrificed for each time. After the last administration,rats were executed under anesthesia to separate serum and strip fat tissue around genitals and kidneys. Finally we inspected the content of GLU,TG,TC,CEBPα,HDL- C,LDL- C and calculated the fat index. Results： Compared with the model group,the index of body weight and fat index in administration groups were significantly reduced and the level of HDL- C was elevated. Among them the levels of TG,TC,LDL- C and CEBPα in medium dose group were reduced most significantly. All the above results were statistically significant（ P 〈 0.05 or P 〈 0. 01）. Conclusions： Huanglian Jiedu Tang has a certain effect on nutritional obesity of rats. Among them the medium dose group is particularly prominent. Considering the effect on majority indexes,the administration time should not be too long except for HDL- C.