超微粉配方颗粒加味柴胡舒肝散对慢性应激性抑郁模型大鼠行为学及HPA轴的影响

Effects of superfine powder formula particles flavored Chaihu Shugan dispersion on behavior and HPA axis of chronic stress depression model rats

目的:探讨加味柴胡疏肝散对慢性应激加孤养抑郁模型大鼠行为学及下丘脑-腺垂体-肾上腺皮质(HPA)轴功能的影响。方法:采用慢性不可预知应激刺激加孤养复制雄性大鼠抑郁模型(21 d)。将成模动物随机分为模型组、氟西汀组、加味柴胡疏肝散超微粉高、中、低剂量组,共5组,每组10只;另取10只正常雄性大鼠作为空白对照组。所有大鼠在造模后第21天开始给药,分别口服灌胃等体积的生理盐水(空白对照组、模型组)或氟西汀(2.1 mg/kg)及加味柴胡疏肝散超微粉药液(生药8.0 g/kg、4.0 g/kg、2.0 g/kg),连续21 d。选用旷场实验得分、动物体重、糖水消耗量等指标衡量各组大鼠行为学改变,采用酶联免疫法检测各组动物药后21 d时血浆CRH及ACTH的含量。结果:加味柴胡舒肝散各组大鼠抑郁样行为显著改善,血浆CRH及ACTH浓度比模型组显著降低,差异具有统计学意义(P<0.01,P<0.05)。结论:加味柴胡疏肝散超微粉配方颗粒可通过抑制HPA轴的功能从而起到抗抑郁的作用。

Objective To investigate the effects of Chaihu Shugan（ CHSG） dispersion,a compound traditional Chinese herbal medicine.On behavior and hypothalamic-pituitary-adrenocortical（ HPA） axis of rats with chronic mild unpredicted stress depression.Method Rats were singly housed and exposed to an unpredicted sequence of continuous 21 days to induce depression.To divide the model animal into 5 groups： untreated groups,fluoxetine group and high CHSG dispersion group,medium CHSG dispersion group,low GHSG dispersion group.Each group had 10 rats.Except thouse groups,taking 10 normal male rats as normal control group.Since the twenty-first day,All rats oral or intragastrically administered with equal volume agents respectively for 21 days normal saline for the normal control group and the untreated group,fluoxetine（2.1mg/kg） for the fluoxetine group and CHSG dispersion（ crude drug 8.0g/kg,4.0g/kg,2.0g/kg）.Behavioral scores of rats were detected by open-field test and sucrose preference test,and the plasma levels of CRH and ACTH in different groups were detected by euzymelinked immunosorbent assay.Results The depressive behaviors of the rats were improved significantly and the levels of plasma CRH and ACTH were obviously reduced in the CHSG dispersion group（ P〈0.01,P〈0.05）.Conclusion CHSG dispersion can restrain HPA hyperactivity of rats cause by chronic stress and has antidepressive effects.