高效液相色谱法同时测定三七伤药片中新北美圣草苷和柚皮苷的含量

Simultaneous determination of neoeriocitrin and naringin in sanqi shangyao tablets by HPLC

目的建立三七伤药片中同时测定新北美圣草苷和柚皮苷含量的方法。方法以甲醇为溶剂,进行超声提取;色谱柱:Agilent C_(18)(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液(17∶83),流速:1 mL·min^(-1),检测波长:283 nm,柱温35℃。结果新北美圣草苷在87.55~1 313.25 ng范围内线性关系良好(r=0.999 4),平均回收率为99.13%,RSD=1.21%(n=6),柚皮苷在80.4~1 206 ng范围内线性关系良好(r=0.999 6),平均回收率为96.78%,RSD=3.34%(n=6)。结论高效液相色谱法(HPLC)可以同时测定新北美圣草苷和柚皮苷的含量,方法简便、准确、灵敏度高、重现性好,可用于三七伤药片中骨碎补的质量控制。

Objective To establish a high performance liquid chromatography （ HPLC ） method for the simultaneous determination of neoeriocitein and naringin in Sanqi Shangyao Tablets. Methods Samples were prepared by ultrasonic extraction with methanol ; the Ag-ilent C18（250 mm×4. 6 mm,5μm） chromatographic column and the mobile phase of acetonitrile-0.1% aqueous phosphoric acid （17-83） were used for isocratic elution with the flow rate of 1.0 mL·min-1 and the detective wavelength at 283 nm. The column tem-perature was set at 35℃ . Results The linear correlation of the neoeriocitrin was good in the range of 87. 55 -1313. 25 ng （ r =0. 9994） ,the recovery rate was 99. 13% and RSD was 1.21% （n =6）. The linear correlation of the naringin was good in the range of 80.4-1206 ng,the recovery rate was 96. 78% and RSD was 3. 34%（n=6）. Conclusions This HPLC method could simultaneously determine the concentrations of neoeriocitrin and naringin. The method is simple, accurate, sensitive and reliable, which can be used for the quality control of Sanqi Shangyao Tablets.