葡萄糖调节蛋白和环氧合酶-2在肝癌组织中的表达及其相关性研究

Expression and its correlation of GRP78 and COX-2in hepatocellular carcinoma tissues

目的研究葡萄糖调节蛋白(GRP78)和环氧合酶-2(COX-2)在肝癌组织中表达及与临床病理资料的关系。方法采用免疫组化法检测144例肝癌组织芯片中GRP78和COX-2的表达情况。结果肝癌组织中GRP78、COX-2的阳性表达率分别为47.22%、56.94%;GRP78与乙肝病毒感染史、丙氨酸氨基转移酶(ALT)差异有统计学意义(P<0.05),但与性别、年龄、肝硬化病史、甲胎蛋白(AFP)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肿瘤大小、分期、分化程度差异无统计学意义(P>0.05),COX-2的表达与肿瘤组织分化程度差异有统计学意义(P<0.025),与性别、年龄、肝硬化病史、乙肝病毒感染史、AFP、AST、ALT、ALP、肿瘤大小、分期差异无统计学意义(P>0.05);COX-2与GRP78蛋白的表达呈正相关(r=0.204,P<0.025)。结论 COX-2的表达与肝癌组织分化程度显著相关,GRP78阳性表达的肝癌组织中COX-2的表达升高,两者在肝癌组织中的关系密切。

Objective To investigate the expression of glucose-regulated protein78 （GRP78） and Cyclooxygenase-2 （COX-2） in hepa-tocellular carcinoma（ HCC） tissues,and their clinical significance. Methods The expression of GRP78 protein and COX-2 were detec-ted by Immunohistochemical staining in 144 cases of hepatocellular carcinoma tissues microarray. Results The positive expression rate of GRP78 and COX-2 in hepatocellular carcinoma tissues were 47.22% and 56. 94% respectively. GRP78 was significantly correlated with HBV infection and ALT（P 〈0.05） ,while it was not related with sex,age,cirrhosis of liver,AFP, AST, ALT, ALP,the size,stage and differentiation of tumor. COX-2 expression was significantly associated with the tumor differentiation （ P 〈0. 025）. No significant difference was found between increased COX-2 and other tested items （P 〉0. 05）. The expression of COX-2 was positively correlated with the expression of GRP78 （r =0.204 ,P〈0.025 ） . Conclusion The expression of COX-2 was correlated with differentiation of hepatocellular carcinoma tissue. COX-2 expression is high in hepatocellular carcinoma which GRP78 expression is positive,so that the relationship between the two are very close.