血尿酸对大鼠动脉粥样硬化发展的作用机制

The mechanism of high serum uric acid in mice model with atherosclerosis progression

目的探讨高血尿酸(SUA)在大鼠动脉粥样硬化发展过程中的作用机制。方法利用线损伤动脉法构建动脉粥样硬化大鼠模型。收集动脉粥样硬化组和健康对照组大鼠空腹心脏血并提取获得血清,用尿酸(UA)试验盒和高敏性C反应蛋白(hs-CRP)快速测定试剂盒分别测定血清样本中的UA和hs-CRP含量水平。动脉粥样硬化中颈动脉内中膜厚度(cIMT)和冠状动脉钙化(CAC)分别采用超声检测仪和多层螺旋CT进行检测,其中冠状动脉钙化积分(CACS)采用Agatston积分法计算。对SUA与hs-CRP、cIMT和CACS进行相关性分析。最后对颈动脉组织进行HE染色并观察有无内膜增厚现象。结果动脉粥样硬化组中SUA和hs-CRP含量水平及cIMT和CACS均值均高于对照组,差异具有统计学意义(P<0.05)。相关性分析结果显示在动脉粥样硬化中SUA与hs-CRP、cIMT和CACS呈正相关。HE染色显示颈动脉组织内膜增厚。结论在大鼠动脉粥样硬化发展中,SUA与hs-CRP、cIMT和CACS呈正相关,高血尿酸可能通过炎症作用影响大鼠动脉粥样硬化的发展。

Objective To study the mechanism of high serum uric acid in mice model with atherosclerosis progression. Methods Ather-osclerosis mice model was made with wire - injury carotid method. Serum samples were collected from atherosclerosis and control group mice after fasting. Uric acid assay and hs - CRP rapid test kits were performed to determine the uric acid and hs - CRP level. Ultrasonographic machine and multi - slice computed tomography were used to test carotid intima - media thickness （ cIMT） and coronary artery calcification （ CAC） , respective-ly. CAC score （CACS） was calculated by Agatston. H＆E staining was used for visualized observation of carotid tissue. Results The levels of SUA, hs - CRP, cIMT and CACS from atherosclerosis group were higher than those of control group （ P 〈0. 05） . SUA had a positive correlation with hs - CRP, cIMT and CACS in atherosclerosis. HE staining showed carotid artery intimal thickening. Conclusion SUA shows a positive cor-relation with hs - CRP, cIMT and CACS in mice with atherosclerosis progression, and it may influence atherosclerosis progression by inflammatory pathway.