大孔树脂法富集黄芩籽种壳总黄酮

Enrichment of Total Flavonoids from Seed Shell of Scutellaria Baicalensis by Macroporous Resin

目的优选富集纯化黄芩籽种壳总黄酮的大孔树脂及工艺条件。方法采用高效液相色谱(HPLC)法测定野黄芩苷含量。以野黄芩苷作指标成分,运用吸附-解吸实验优选大孔树脂型号;以单因素设计和正交实验考察大孔树脂富集纯化黄芩籽种壳总黄酮工艺。结果 AB-8型大孔树脂吸附-洗脱效率最高,较理想的纯化工艺条件为:上样浓度30.0 g·L^(-1),最佳吸附量0.3 g·g^(-1),吸附流速1.0 m L·min^(-1),树脂柱径高比1∶3,除杂需纯化水量20 BV,5 BV 70%乙醇洗脱。结论该工艺条件下AB-8型大孔树脂富集纯化黄芩籽种壳总黄酮效率较高,工艺条件科学、简便,重复性好。

Objective To optimize enrichment and purification technology of total flavonoids from seed shell of Scutellaria baicalensis by macroporous resin. Methods Scutellarin was selected as the index, and its content was determined by HPLC. Types of macroporous resin were optimized by static adsorption elution tests. Enrichment and purification technology was investigated by orthogonal test and single-factor tests. Results AB-8 macroporous resin showed better effects than others. Optimum purification condition was as follows ： sample concentration was 30. 0 g · L^- 1,the best adsorption capacity was 0. 3 g·g^-1,adsorption velocity was 1. 0 mL·min ^-1 , diameter- height ratio was 1： 3 , the eluting agent was 20 BV purified water, with the total polyphenols being desorbed by 5 BV of 70% ethanol. Conclusion The technology is scientific, simple and highly reproducible. It can be applied to enrich and purify total flavonoids from seed shell of Scutellaria baicalensis by macroporous resin.