Meso-5,10,15,20-四(4-甲氧基苯基)卟啉荧光熄灭法测定铅

A Fluorescence Quenching Method for the Determination of Lead with Meso-(4-Methyloxyphenyl) Porphyrin

基于铅对荧光试剂 Meso- 5 ,10 ,15 ,2 0 -四 (4 -甲氧基苯基 )卟啉 (TMOPP)的荧光熄灭 ,建立了测定微量铅的荧光分析方法。在 p H9.5的 Tris- HCl缓冲体系中 ,最大激发波长和发射波长分别为 4 2 4 nm和6 5 7nm,测定铅浓度的线性范围为 5 .5 0× 10 -7— 7.5 0× 10 -5mol/ L,检出限为 8.5 0× 10 -8mol/ L。用于环境水样中铅含量的分析 ,结果满意。

A new and sensitive fluorimetric method for the determination of lead is established based on quenching effect of lead on the fluorescence of Meso-(4-Methyloxyphenyl) Porphyrin(TMOPP) in pH 9.5 Tris/HCl buffer solution.The maximum excitation and emission wavelengths of TMOPP are 424nm and 657nm,respectively.The linear range of the determination is 5.50×10 -7 - 7.50 ×10 -5 mol/L.The detection limit of lead is 8.50×10 -8 mol/L.This method was used to determine micromount of lead in water samples with satisfactory results.