奥美拉唑与氯冉酸的荷移反应及其测定

Spectrophotometric Determination of Omeprazole Based on the Charge Transfer Reaction between Omeprazole and Chloranilic Acid

奥美拉唑 (电子给予体 )与氯冉酸 (电子接受体 )在丙酮介质中生成 1∶ 1型荷移络合物 ,在波长5 2 5 nm处有最大吸收 ,表观摩尔吸光系数是 1.0 2× 10 3 L· mol-1·cm-1。依据此荷移反应建立了一种快速测定奥美拉唑的荷移分光光度法。药物浓度在 2 0— 30 0 mg/ L范围内服从比耳定律。 6次测定结果的相对标准偏差为 0 .4 8% ,利用本法测定了奥美拉唑胶囊中有效成分的含量 ,结果令人满意。

A rapid spectrophotometric method for the determination of omeprazole was developed based on the 1∶1 charge transfer complex between omeprazole as donor and chloranilic acid as acceptor in acetone with the apparent molar absorptivity of 1.02×10 3L·mol -1 ·cm -1 at 525nm.Beer's law is obeyed in the range of 20—300mg/L of omeprazole.The average standard deviation of six determinations is 0.48%.The method was applied to the determination of omeprazole in capsules with satisfactory results.