反相高效液相色谱法测定口香糖中的抗氧化剂BHA

Determination of BHA in Chewing Gum with High Performance Liquid Chromatography

采用 Zorbax XDB- C18柱 (4 .6× 15 0 mm) ,以乙腈和水按 6 0∶ 4 0的配比作流动相 ,流量为 1m L /min,柱温 30℃ ,检测波长 2 80 nm,建立了一种用反相高效液相色谱测定口香糖中 BHA的方法。检出限为0 .15 mg/kg,回收率在 83%— 10 1%之间 ,相对标准偏差小于等于 7.5 %。本法具有操作简便 ,灵敏、准确 ,重现性好等优点。适用于口香糖中 BHA的定量分析。

A reversed-phase HPLC method was developed to determine BHA in chewing gum with a Zorbax XDB-C 18 column(4.6×150mm) and a mobile phase of CH 3CN∶H 2O(60∶40) at 1.0mL/min of flow rate and 30℃.The detection wavelength was 280nm.The detection limit is 0.15mg/kg, with the RSD of less than 7.5% and the recovery ratio in the range of 83%—101%.This method is convenient,sensitive with good accuracy and precision,and used in the quantitative analysis of BHA in chewing gum.