磺硝酚偶氮若丹宁固相萃取光度法测定生物样品中的汞

Solid Phase Extraction and Spectrophotometric Determination of Mercury in Biological Sample with 4-Nitro-6-Sulfophenolazorhodanine

在 p H2 .0的氯乙酸 -氢氧化钠缓冲介质与吐温 - 80存在下 ,磺硝酚偶氮若丹宁 (NSPAR)与汞反应生成 2∶ 1稳定络合物 ,可被 Waters Sep- Pak C18小柱固相萃取 ,氮 -氮二甲基甲酰氨 (DMF)洗脱后光度法测定 ,λmax=5 5 0 nm,体系摩尔吸光系数ε=8.12× 10 4L· mol-1· cm-1。汞含量在 0 .0 5— 4 .0μg/m L内符合比耳定律 ,用于生物样品中汞含量的测定 。

In pH=2.0 buffer solution in the presence of Tween-80,4-nitro-6-sulfophenolazorhodanine(NSPAR)reacts with mercury (Ⅱ) to form a stable 2∶1 complex.This complex can be extracted by Waters Sep-Park-C 18 cartridge and eluted the retained chelate by DMF.The molar absorptivity is 8.12×10 4L·moL -1·cm -1 at 550nm.Beer's law is obeyed in range of 0.05—4.0μg/mL.This method can be applied to the determination of mercury in biological sample with satisfactory results.