滩羊多胎基因(FecB)TaqMan探针SNP分型与产羔数的关联研究

Correlation Analysis between Fecundity Gene (FecB) SNP Genotyping by TaqMan Probe and Litter Size in Tan Sheep

为了建立滩羊FecB基因SNP高效分型的方法,试验选用373只滩羊利用TaqMan探针FecB基因进行SNP分型研究。结果表明,FecB基因具有3种基因型,分别是XX(87%)、XY(12%)和YY(1%)。FecB基因YY基因型的平均产羔数为3.81只,XY基因型为2.44只,XX基因型为2.01只。FecB基因可以作为滩羊多胎性选育的分子遗传标记,并能有效提高滩羊个体平均产羔数;通过基因型选育,还可以加快滩羊高繁品系的建立。

In order to establish a highly efficient method for SNP genotyping of FecB gene in Tan Sheep,the SNP genotyping of FecB gene from 373 Tan Sheep were conducted using TaqMan probe. The results showed that there were three genotypes in the FecB gene： 87% of wild type（XX）,12% of heterozygous type（XY） and 1% of homozygous mutant type（YY）,and their average litter size was 2.01,2.44 and 3.81,respectively. The combined data showed that the FecB gene can be used as a molecular genetic marker for selection of fecundity traits of Tan Sheep and the presence of certain genotype of FecB gene indicates the higher litter size; the establishment of high-prolificacy varieties of Tan Sheep may be accelerated by FecB genotype selection.