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PCV2-Cap蛋白在大肠杆菌中的可溶性表达与鉴定 被引量:1

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摘要 pCold是一种新型载体,可以在大肠杆菌中高效表达可溶性的目的蛋白,将含有Cap蛋白基因的重组质粒pcold-ORF2转化大肠杆菌,可溶性表达Cqp蛋白,通过SDS-PAGE和Western blot对融合蛋白进行鉴定,大量表达后,对其进行了纯化。
出处 《河南畜牧兽医(综合版)》 2017年第4期5-6,共2页 Henan Journal of Animal Husbandry and Veterinary Medicine
关键词 PCV2 大肠杆菌
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