乳化剂Span 80对乳液静电纺PCL纤维担载盐酸四环素能力的影响

Influence of Span 80 on Drug Loading Capability of Emulsion Electrospun PCL Fibers Loaded with Tetracycline Hydrochloride

以高聚物聚己内酯(PCL)溶解在三氯甲烷中作为油相,水溶性盐酸四环素(Tet)溶解在蒸馏水中作为水相,加入不同质量的失水山梨糖醇单油酸酯(Span 80)作为乳化剂,利用乳液静电纺丝制备皮芯结构的PCL/Tet载药纤维.观察纤维形貌、纤维内部Tet的分布情况和皮芯结构形成,计算纤维载药量和包封率,研究Span 80对纤维担载Tet能力的影响.研究结果表明:不加Span 80的乳液制备的PCL/Tet载药纤维膜具有很低的载药量和包封率,Span 80质量分数在0.5%~2.0%内,纤维具有完整的皮芯结构,药物被包裹在纤维芯层;随着Span 80质量分数的增加,纤维的皮层厚度有增加的倾向,芯层的药物减少,纤维的载药量和包封率则下降.

Polycaprolactone（PCL） fibers containing water-soluble drug tetracycline hydrochloride（Tet） were fabricated by emulsion electrospinning, in which PCL was dissolved in the chloroform as the oil phase and water soluble tetracycline hydrochloride was dissolved in distilled water as the water phase. Sorbitan monooleate（Span 80） as an emulsifier was added in the solution with different mass fractions. Fiber morphology, distribution of Tet in the fibers and formation of core-sheath structure were observed. Drug loading and encapsulation efficiency were calculated to study the influence of Span 80 mass fraction on drug loading capability of fibers. The results indicate that fibers without Span 80 have quite low drug loading and encapsulation efficiency. With the mass fraction of Span 80 ranged from 0.5% to 2.0%, fibers with integral core-sheath structure are obtained, and Tet is well encapsulated into the core region of the fibers. With the increase of Span 80 mass fraction, the thickness of fiber sheath increases and drug loaded in the core region of fibers decreases. Therefore, fibers＇ drug loading and encapsulation efficiency decrease.