蟾毒灵对大鼠吗啡成瘾消退及其重建的影响

The Effects of Bufalin on Extinction and Reinstatement of Morphine Addictive Rats

目的研究蟾毒灵在吗啡成瘾消退和重建中的药理作用。方法大鼠随机分为溶酶组和蟾毒灵组,每组各12只。腹腔注射盐酸吗啡并通过条件性位置偏爱装置建立吗啡成瘾模型后,2组分别注射溶酶和蟾毒灵(0.6mg/kg)并检测其偏爱分值变化。结果蟾毒灵组在注射蟾毒灵后,偏爱分值降低,与后测值比较,差别有统计学意义(P<0.05),而且效应持续3d以上;溶酶组的偏爱分值与后测值比较,差别无统计学意义(P>0.05)。成瘾消退后采用半剂量吗啡注射重建吗啡成瘾,蟾毒灵组和溶酶组的偏爱分值比较,差别无统计学意义(P>0.05)。结论蟾毒灵可促进大鼠吗啡成瘾的消退,但不影响成瘾消退后的重建。

Objective To study the pharmacological actions of bufalin on extinction and relapse oi morphine addictive rats. Methods We established the rat models of morphine addiction by using condi- tioned place preference. To explore the effects of bufalin on extinction and reinstatement of morphine ad- dictive rats, the rats in the experimental group were given a single peritoneal injection of bufalin （0.6 mg/kg） after addiction while the control group only received an injection of same amount of DMSO. Results The preference value decreased significantly compared with the post-test value（P〈0.05） after bufalin injection and the effects lasted at least 3 days. However, the preference value of the control group didn＇t change significantly after injection of DMSO. Then half-dose of morphine was injected to reinstate conditioned place preferences after extinction. The results indicted both groups produced reinstatement and there was no significant difference between the bufalin group and the DMSO group. Conclusion Bu- falin promotes the extinction of morphine addictive rats, while it does not affect half-dose of morphine re- instatement.