摘要
[目的]优化高活性纳豆激酶的纳豆生产工艺条件。[方法]从市售纳豆产品中分离筛选出高活性的菌株作为试验菌株,采用固态发酵的方法制备纳豆,对影响纳豆激酶产量的主要因素浸泡时间、接种量、发酵时间等进行考察,研究高活性纳豆激酶的工艺条件。[结果]利用对甲基苯磺酰L精氨酸甲酯(TAME)法测定纳豆激酶活性,确定最佳纳豆激酶酶活的纳豆生产工艺条件为浸泡时间8 h、接种量10%、发酵时间48 h。[结论]该研究可为高活性纳豆激酶的生产提供参考。
[ Objective] To optimize the process conditions of high activity natto kinase. [ Method] Natto was isolated from commercial natto prod- ucts and used as the experimental strain. Natto was prepared by solid-state fermentation. The process conditions of high activity natto kinase were studied. The main factors affeeting the yield of natto kinase were as follows :immersion time ,inoculation amount and fermentation time. [ Result ] The activity of natto kinase was determined by TAME method. The optimum natto kinase production process conditions were determined as 8 h soaking time,10% inoculation amount,48 h fermentation time. [Conclusion] The study can provide reference for production of high activity natto kinase.
作者
张浩
王家林
ZHANG Hao WANG Jia-lin(Biological Engineering Lab of Qingdao University of Science and Technology, Qingdao, Shandong 26604)
出处
《安徽农业科学》
CAS
2017年第10期93-94,共2页
Journal of Anhui Agricultural Sciences
关键词
纳豆
纳豆激酶
活性测定
工艺优化
Natto
Natto kinase
Activity determination
Process optimization