高活性纳豆激酶工艺改进研究

Study on the Improvement of High Activity Natto Kinase

[目的]优化高活性纳豆激酶的纳豆生产工艺条件。[方法]从市售纳豆产品中分离筛选出高活性的菌株作为试验菌株,采用固态发酵的方法制备纳豆,对影响纳豆激酶产量的主要因素浸泡时间、接种量、发酵时间等进行考察,研究高活性纳豆激酶的工艺条件。[结果]利用对甲基苯磺酰L精氨酸甲酯(TAME)法测定纳豆激酶活性,确定最佳纳豆激酶酶活的纳豆生产工艺条件为浸泡时间8 h、接种量10%、发酵时间48 h。[结论]该研究可为高活性纳豆激酶的生产提供参考。

[ Objective] To optimize the process conditions of high activity natto kinase. [ Method] Natto was isolated from commercial natto prod- ucts and used as the experimental strain. Natto was prepared by solid-state fermentation. The process conditions of high activity natto kinase were studied. The main factors affeeting the yield of natto kinase were as follows ：immersion time ,inoculation amount and fermentation time. [ Result ] The activity of natto kinase was determined by TAME method. The optimum natto kinase production process conditions were determined as 8 h soaking time,10% inoculation amount,48 h fermentation time. [Conclusion] The study can provide reference for production of high activity natto kinase.