西地碘粉的急性毒性研究

Study on the Acute Toxicity of Cydiodine Powder

[目的]研究西地碘粉对小鼠的经口急性毒性。[方法]选用清洁级ICR小鼠60只,采用寇氏法进行急性毒性试验,观察小鼠西地碘粉急性中毒症状,并测定西地碘粉经口给药对小鼠的LD_(50)。[结果]小鼠经口给药后的急性中毒症状主要包括:给药后15 min,高剂量组小鼠出现精神沉郁,被毛粗乱无光,并逐渐转入抑制,反应迟钝、不食、麻痹。0.5~1.0 h后有小鼠出现呼吸困难,伴随脖颈伸直、食欲废绝;3 h后开始出现死亡,小鼠死亡前出现惊厥、抽搐、震颤等神经症状。急性死亡发生于给药后3~24 h。中毒死亡后剖检发现中毒症状主要表现为对肝脏的损伤,其次是肺和心脏。根据各剂量组死亡率,计算出西地碘粉对小鼠的急性经口LD_(50)为4 972.75 mg/kg.,其LD_(50)95%可信限为4 382.77~5 642.16 mg/kg。[结论]西地碘粉对小鼠的经口LD_(50)介于501~5 000 mg/kg,属于低毒性药物。

[Objective] To study theoral acute toxicityof cydiodine powder on mice. （Method] 60 clean-level ICR mice were selected to make acute toxicity test by using Korbor method. The acute toxicity symptoms of cydiodine powder on mice were observed, and its LD50 on mice by oral administration was determined. [ Result]The acute toxicity symptoms of cydiodine powder on mice were as follows： after drug administration 15 min, mice in high-dose group had depressed spirit, disordered clothing hair, slow response, paralysis and loss of appetite; Mter drug administration 0.5 - 1.0 h, mice had difficult breathing, accompanied by straight neck and loss of appetite; After drug administration 3 h, mice began to die and eclampsia, convulsion, tremor and other neural symptoms appeared before the death of mice. And the acute death occurred after drug administration 3 - 24 h. It was observed through autopsy that the main manifestation of poisoning symptoms was the damage to liver, followed by the damage to lung and heart. According to the death rate in each dose group, the acute oral LD50 of cydiodine powder on mice was 4 972.75 mg/kg, 95% confidence limit of LD50 was 4 382.77 - 5 642. 16 mg/kg. [ Conclusion] The oral LD50 of cydiodine powder on mice was between 501 - 5 000 mg/kg, belonging to lowtoxicity drug.