孕中期联合AFP、Free β-HCG、uE_3检测的产前筛查意义

Significance of prenatal screening for AFP,Free β-HCG and uE_3 in the second trimester of pregnancy

目的探讨孕中期联合AFP、Free β-HCG、uE_3检测在产前筛查中的临床意义。方法采用时间分辨免疫荧光法对2825例孕中期孕妇血清的AFP、Free β-HCG和uE_3浓度进行联合检测,结合孕周、体重、年龄等因素进行风险评估,并与羊水染色体分析结果进行对照。结果 2825例孕妇中共筛查出高危妊娠133例,其中21-三体高风险124例,18-三体高风险者9例,两者筛查高风险率分别为4.71%、0.32%。经染色体培养核型分析,共确诊染色体异常患者11例,其中21-三体患儿2例,18-三体患儿1例,其他染色体异常8例。结论孕中期联合AFP、Free β-HCG、uE_3检测可有效检出常见染色体异常患儿,具有重要的产前筛查意义。

Objective：To investigate the value of the second trimester screening of Down syndrome for the chromosomal abnormality. Methods：To use the time-resolved immunofluorescence to detect the serum AFP,Free β-HCG and uE3 in the second trimester of 2825 cases of pregnant women,combined with gestational age,weight,age and other factors,and assess the risk through risk assessment software.The screening results were compared with the results of amniotic fluid chromosome analysis. Results：133 cases of high-risk pregnancys were screened out in the 2825 cases of pregnant women,including 124 cases of high risk of trisomy 21,9 cases of high-risk of trisomy 18,the high-risk rate of trisomy 21 and trisomy 18 are 4.71%、0.32% respectively. After the chromosome karyotype analysis,a total of 11 cases of patients were confirmed diagnosed,including 2 cases of trisomy 21,1 cases of trisomy 18,and 8 cases of other chromosomal abnormalities. Conclusion：Second trimester screening of Down syndrome is an ideal screening mode,it can effectively detect the common chromo somal abnormalities in children,and reduce the risk of birth defects.