人类卵裂期胚胎程序化和玻璃化冻融方法的临床效果分析

Analysis of clinical outcomes and frozen recovery of human cleavage stage embryos with programmed freezing and vitrification

目的回顾性分析比较慢速程序化和快速玻璃化方法在冻融人类卵裂期胚胎中的效果和临床结局。方法对1386例程序化冻融周期和8745例玻璃化冻融周期的相关资料进行统计学分析,评价参数主要有胚胎复苏率,完整率,取消率,临床妊娠率以及种植率等各项指标。结果玻璃化冻融后的胚胎复苏率,完整率,临床妊娠率以及种植率明显优于程序化冻融法,且周期取消率显著低于程序化方法。结论相比于经典的程序化冻融方法,玻璃化冻融方法更适合辅助生殖治疗中的早期卵裂期胚胎的冻存。

Objective：Retrospective analysis of outcomes and effects of freeze-thawing human cleavage stage embryos by a slow programmed freezing and fast vitrification method. Methods：Statistical analysis of 1386 cases of programmed freezing and thawing cycles and the related data of 8745 cases of vitrification cycles was performed,looking at the following evaluation parameters：embryo recovery rate,completion rate,cancellation rate,clinical pregnancy rate,implantation rate,and other indicators. Results：The recovery rate,completion rate,clinical pregnancy rate,and implantation rate in the vitrification group was significantly higher than the group which underwent the programmed freezing and thawing method. The comparative cycle cancellation rate was significantly lower in the vitrification group. Conclusion：Compared with the traditional programmed freeze and thaw method,vitrification is more effective for assisted reproduction treatment of cryopreserved early cleavage stage embryos.