摘要
通过单因素试验和正交试验对甘蔗糖蜜培养饲用酵母菌YS-01的条件进行了初步优化。结果表明,饲用酵母菌YS-01在处理糖蜜中生长良好,优化的培养条件是:糖蜜浓度为100Bx、接种量为8.0%、培养时间为12 h;最佳营养盐配比为:硫酸铵0.10%、尿素0.10%、磷酸二氢钾0.16%、七水硫酸镁0.05%;该条件下OD值达到14.670,活菌数达到2.55×10~8CFU/m L。
In the present study,the single factor experiment and orthogonal experiment were used to optimize the cultivation conditions for feed yeast strain YS-01 with sugarcane molasses. The results revealed that the feed yeast strain showed good growth in the treated molasses; the optimal culture conditions were： molasses concentration was 10^0 Bx, inoculation amount was 8.0%,fermentation duration was 12 h; the optimum concentrations of various nutrients were：（NH4）2SO4 was 0.10%, CO（NH2）2 was 0.10%, KH2PO4 was 0.16%, MgSO4·7H2O was 0.05%. Under this condition, the OD value reached 14.670 and the cell density reached 2.55×10^-8CFU/m L.
出处
《畜牧与饲料科学》
2017年第2期26-28,共3页
Animal Husbandry and Feed Science
关键词
甘蔗糖蜜
饲用酵母菌
培养条件
优化
OD值
活菌数
sugarcane molasses
feed yeast
culture conditions
optimization
OD value
cell density
