体细胞核移植技术的研究进展

Progress in Somatic Cell Nuclear Transfer

体细胞核移植转基因法效率高、成本低,目前已经成为构建转基因动物极为重要的方法。但该项技术的成功率较低,而且许多克隆动物会出现胎儿过度生长、出生前死亡、出生后生长异常等病理反应。文章以核移植生物学理论为基础,重点探讨了改进型核移植技术(OSM)在操作方法方面的改进研究,分析了影响核移植成功率的影响因素,为核移植技术的进一步完善提供了理论基础。

The somatic cell nuclear transfer （SCNT） has been widely applied in production of transgenic animal due to the high performance and the low cost. However, cloning by somatic cell nuclear transfer（SCNT） is still inefficient. This paper summarized some important aspects of OSM, and also analyzed the factor that related to the OSM.