银杏叶提取物对大鼠创伤性颅脑损伤后MMP-2和MMP-9的影响

Effect of Ginkgo biloba extract on expressions of MMP-2 and MMP-9 after traumatic brain injury in rats

目的为探讨银杏叶提取物(GBE)对大鼠创伤性颅脑损伤后(TBI)脑水肿及MMP-2、MMP-9蛋白表达的影响。方法采用Feeney法建立大鼠TBI模型。80只健康雄性成年SD大鼠,采用随机数字表法分为4组(n=20):假手术组(Sham组)、TBI组、TBI+GBE组和TBI+强力霉素(DOX)组。于建模后1、3、7、14 d进行改良神经功能评分(m NSS);采用干湿重法测量损伤区脑组织含水量;免疫荧光和Western blotting测定脑组织中MMP-2、MMP-9蛋白表达变化。结果与Sham组比较,其他3组脑组织含水量和m NSS评分均显著升高(P<0.05),脑组织中MMP-2和MMP-9蛋白表达上调(P<0.05);与TBI组比较,TBI+GBE组和TBI+DOX组脑组织含水量和m NSS评分显著降低(P<0.05),脑组织MMP-2和MMP-9蛋白表达下调(P<0.05)。结论 GBE可减轻大鼠TBI后脑水肿,改善损伤后神经功能,其机制可能与抑制伤后脑组织MMP-2、MMP-9的表达相关。

Objective To investigate the effect of Ginkgo biloba extract （GBE） on expression of matrix metalloproteinase-2 （MMP-2） and MMP-9 after traumatic brain injury （TBI） in rats. Methods TBI animal models were established by Feeney method. Eighty SD rats were randomly and equally divided into 4 groups：sham operation group （Sham group）, TBI group, TBI＋GBE group and TBI＋doxycycline （DOX） group. The modified neurologic severity score （mNSS） was tested on days 1, 3, 7 and 14 after modeling. Brain water content was measured by wet-dry weight method. The MMP-2 and MMP-9 expressions were determined by immunohisto-chemical staining and Western blotting. Results Compared with the Sham group, the levels of brain water content and mNSS significantly increased （P〈0.05）, and the protein expressions of MMP-2 and MMP-9 were up-regulated in others 3 group （P〈0.05）. Compared with the TBl group, brain water content and mNSS significantly decreased （P〈 0.05）, and the protein expressions of MMP-2 and MMP-9 were down-regulated in TBI＋GBE group and TBI＋DOX group （P〈0.05）. Conclusions GBE can reduce brain edema and improve neurological function after TBI, and its mechanism may be related to inhibiting MMP-2 and MMP-9 expressions.