白芨愈伤组织诱导研究

Study on Induction of Bletilla Striata Callus

目的:以白芨假鳞茎为培养材料,进行愈伤组织诱导和抗褐化研究.方法:使用兰科常用培养基MS,B5,KC分别作为基础培养基,应用正交试验方法设计生长调节剂配比.结果:当基础培养基为MS培养基时,并且6-BA1.5mg/L+2,4-D3.0mg/L时,愈伤组织诱导率最高,达73.3%.另外,添加活性炭可防止外植体褐化:活性炭含量为1.5g/L时,褐化率为44%,愈伤组织诱导效果最好;活性炭含量为3.0g/L时,褐化率最低为11%,愈伤组织诱导效果不好.结论:白芨愈伤组织诱导的最佳培养基为MS+6-BA1.5 mg/L+2,4-D3.0mg/L+AC2 g/L.

Objective：In order to study the anti-browning and induction of Bletilla striata callus by tak- ing the false bulbs of Bletilla Striata as tissue culture material. Methods：The medium of MS, B5 and KC were selected as the basic culture medium of orchidaceae respectively. The ratio of growth regula- tors was adjusted via orthogonal tests. Results：The induction ratio was the maximum of 73.3% when MS medium was used as basic culture medium with 6-BA 1.5 mg/L ＋ NAA 0mg/L ＋ 2,4-D 3.0 mg/L. Additionally, addition the activated carbon into the basic culture medium could prevent Bletilla striata browning. The browning rates were 44% and 11% when the contents of activated carbon were 1.5 g/ L and 3.0g/L. The induction efficacy of Bletilla striata callus was the highest and least for both cont- ensts respectively. Conclusion：The optimal medium for induction of callus was MS ＋ 1.5mg/L of 6-BA ＋ 0mg/L of NAA ＋3.0 mg/L of 2,4-D ＋2g/L of AC.