补肾活血汤对腰椎间盘退变大鼠TNF-α表达的影响

Effect of kidney-tonifying and blood-activating decoction on TNF-α expression in rats with lumbar disc degeneration

目的:探讨补肾活血汤对腰椎间盘退变大鼠TNF-α表达的影响,为延缓或阻止椎间盘退变提供客观评价指标。方法:将72只大鼠随机分为空白组、模型组、中药组,每组24只,采用皮下注射D-半乳糖法建立衰老大鼠椎间盘退变模型,造模成功后中药组予补肾活血汤灌胃,空白组与模型组予蒸馏水灌胃。连续给药4周后以Elisa法检测大鼠椎间盘组织中TNF-α的含量,以光密度OD值表示。结果:与空白组比较,模型组TNF-α含量明显升高,差异有统计学意义(P<0.05);中药组TNF-α含量低于模型组,两组比较,差异有统计学意义(P<0.05)。结论:补肾活血汤可能是通过抑制大鼠退变腰椎间盘组织中TNF-α的表达来减缓椎间盘的退变过程。

Objective ： To investigate the effect of kidney -tonifying and blood - activating decoction （KBD） on the expression of tumor necrosis factor- alpha（TNF -c~）in rats with lumbar disc degeneration, and to provide as- sessment indices for delaying or preventing lumbar disc degeneration. Methods： Seventy - two rats we,＇e randomly and equally divided into blank group, model group, and traditional Chinese medicine （TCM）group. Subcutaneous in- jection of D - galactose was used to establish an aging rat model of lumbar disc degeneration. The TCM group was giv- en KBD by gavage,while the blank group and model group were given distilled water by garage. After 4 weeks of ad- ministration,ELISA was used to measure the content of TNF -o~ in the intervertebral disc tissue,as represented by op- tical density. Results：Compared with the blank group, the model group had significantly increased TNF - a content （P 〈0. 05）. The TCM group had significantly lower TNF-c~ content than the model group （P 〈0.05 ）. Conclu- sion：KBD can delay lumbar disc degeneration,possibly by suppressing the TNF -cx expression in degenerative in- tervertebral disc tissue.