^(60)Coγ射线对大鼠涎腺功能形态的影响

Effect of ^(60)Coγ rays on the function and morphology of salivary gland in rats

目的:观察^(60)Coγ射线对大鼠涎腺功能及形态学影响。方法:取成年雄性SD大鼠48只随机分为实验组和对照组各4组,每组各6只。实验组大鼠给予一次性^(60)Coγ射线照射头颈部后,每6 d称重,并分别于1、4、8、12周后,取全血以观察其全身情况,及唾液、腮腺组织以观察涎腺功能、形态学变化。结果:经^(60)Coγ18 Gy照射后,大鼠体重第6 d左右降到最低后逐渐增加;1周后白细胞计数明显升高,红细胞计数及血红蛋白略有升高,随后各项指标均回归至正常值范围内。唾液流量及唾液淀粉酶含量虽较对照组明显降低,但有增加趋势(P<0.05)。与对照组相比较,实验组腮腺腺泡均有变小趋势,腮腺组织中AQP5表达量随时间呈负相关性(P<0.05)。结论:^(60)Coγ射线18Gy放射后对大鼠涎腺功能及形态变化明显。

Objective：To investigate the effect of 60 Coγ rays on the function and morphology of salivary gland in rats.Methods：Forty-eight adult male SD rats were randomly divided into 1,4,8 and 12 weeks for control and experimental groups,respectively.There were 8 groups that each group with 6 rats.After experimental groups were radiated with 60Coγrays on head and neck region in dose of 18 Gy,the rats were weighted every 6 days and taken the whole blood ,that the parotid gland tissue and saliva of them were tested .Results：After exposure to 60Coγrays,the weight,white blood cell count,red blood cell count and hemoglobin of rats decreased to the lowest after 6 days and then gradually increased .Even though there was an increasing trend on saliva flow and salivary amylase content , the experimental groups were lower than the control groups significantly （ P〈0.05 ） .The acinar cells of experimental groups tended to become smaller , and the expression of AQP5 in the parotid gland was negatively correlated with the time in the experimental groups （P〈0.05）.Conclusion：60Coγrays have a little impact on the whole body ,but a great impact on salivary gland function and morphology .