益气除痞汤对酸性反流性食管炎模型大鼠TNF-α及IL-6的影响

Effect of Yiqi Chupi Tang on TNF-α and IL-6 of Model Rat with Acid RE

目的:观察益气除痞汤对酸性反流性食管炎(RE)模型大鼠食管组织与血浆肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)水平的影响,探讨益气除痞汤治疗RE的作用机制。方法:60只大鼠随机分成假手术组、模型组、益气除痞汤高剂量组、益气除痞汤低剂量组、多潘立酮组,每组10只。除假手术组外,其他各组大鼠采用不全幽门结扎及食管下括约肌切开术制备酸性反流性食管炎模型,造模第2天,益气除痞汤高、低剂量组、多潘立酮组分别按50 g/kg、10 g/kg、4.5 mg/kg给予相应的药物,假手术组及模型组给予等体积生理盐水,每天分2次灌胃,给药7天。取血液以及食管标本,采用ELISA法测定大鼠食管与血浆中TNF-α以及IL-6的含量。结果:与假手术组比较,模型组大鼠食管组织、血浆中TNF-α及IL-6的含量显著升高,差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,益气除痞汤高、低剂量组、多潘立酮组大鼠食管组织、血浆中TNF-α及IL-6的含量显著降低,差异均有统计学意义(P<0.01)。与益气除痞汤低剂量比较,益气除痞汤高剂量组大鼠食管组织、血浆中IL-6的含量降低的更明显,差异均有统计学意义(P<0.05,P<0.01)。结论:益气除痞汤能够明显降低酸性反流性食管炎模型大鼠食管组织、血浆中TNF-α及IL-6的含量,通过降低TNF-α及IL-6的表达,改善食管局部及外周炎症反应。

Objective. To observe the effect of Yiqi Chupi tang on edible tissues and levels of tumor necrosis factor-α （TNF-α）and Interleukin-6（IL-6）of model rats with acid reflux esophagitis（RE）, and its mechanism for the treatment of RE. Methods. Selected 60 rats and divided them into sham operation group, model group, high dose of Yiqi Chupi tang group, low dose of Yiqi Chupi tang group, domperidone group randomly, 10 cases in each. Except sham operation group, every group was given partial pylori ligation and lower esophageal sphincterotomy to make model rats with acid RE. After the second day of creation of models, high dose of Yiqi Chupi tang group, low dose of Yiqi Chupi tang group and domperidone group were given corresponding drug of 50 g/kg, 10 g/kg, 4.5 mg/kg respectively, and the sham operation group and model group were given equal volume saline, gavage twice a day and administration for a week. The specimens of blood and oesophagus were extracted, and contents of TNF-α and IL-6 in the oesophagus and plasma of rats were detected with enzyme-linked immunosorbent assay. Results： Comparing with those in the sham operation group, contents of TNF-α and IL-6 in the oesophagus tissues and plasma of rats in model group were increased significantly（P 〈 0.05, P〈 0.01）. Comparing with those in the model group, contents of TNF-α and IL-6 in the oesophagus tissues and plasma of rats in high dose of Yiqi Chupi tang group, low dose of Yiqi Chupi tang group, domperidone group were decreased significantly（P 〈 0.01）. Comparing with that in low dose of Yiqi Chupi tang group, decrease of contents of TNF- α and IL-6 in the oesophagus tissues and plasma of rats in the high dose of Yiqi Chupi tang group was more obvious（P 〈 0.05, P〈 0.01）. Conclusion. Yiqi Chupi tang can reduce contents of TNF-α and IL-6 in the oesophagus tissues and plasma significantly, and improve local and peripheral inflammatory response of oesophagus by reducing the expression of TNF-α and IL-6.