国外水稻种质资源抗稻瘟病基因Pi-ta和Pi-b的分布和基因型分析

Distribution and Genotypic Analysis of Blast Resistance Genes Pi-ta and Pi-b in Foreign Rice Germplasm Resources

稻瘟病抗性基因Pi-ta和Pi-b具有持久稳定的稻瘟病抗性,被广泛用于中国的水稻育种和生产。本研究通过Pi-ta、Pi-b的功能标记,对搜集到的国外60份水稻种质资源进行分子检测和分析。研究表明,抗病基因Pi-ta和Pi-b在60份材料有一定的分布,Pi-ta的分布频率要大于Pi-b。四种基因型中,pi-ta/pi-b的分布频率最高,为91.6%,Pi-ta/pi-b分布频率为6.7%,pi-ta/Pi-b分布频率为1.7%,没有检测到两个抗病基因都携带的品种。

Pi-ta and Pi-b have been widely used in rice breeding and commercial production in China for their lasting and stable resistance. In this study, 60 foreign rice germplasms were detected using the functional markers of Pi-ta/pi-ta and Pi-b/pi-b alleles. The results showed that the resistant genes Pi-ta and Pi-b were disturbed in 60 rice germplasms, and the distribution frequency of Pi-ta was higher than that of Pi-b. In four genotypes, the percentage of pi-ta/pi-b was the highest, accounting for 91.6%, in 60 varieties, and other two genotypes were Pi-ta/pi-b and pi-ta/Pi-b, accounting for 6.7% and 1.7% of total, respectively, and not found the Pi-ta/Pi-b genotype in all rice germplasms.