苦豆子种子萌发期及幼苗期对盐、旱耐受性的探索

The Study of Sophora alopecuroides Seeds and Seedling Drought and Salt Tolerance

苦豆子因其具有较强的耐盐、耐旱特性常被用于沙漠地区的防风固沙材料。本实验采用浓度为0.2 mol/L、0.3 mol/L、0.4 mol/L、0.5 mol/L的NaCl溶液模拟不同程度的盐胁迫,用PEG-6000质量分数为5%、10%、15%、20%、25%的溶液(对应的渗透势分别为-0.054 MPa,-0.177 MPa,-0.393 MPa,-0.735 MPa,-1.25 MPa)模拟不同程度的干旱胁迫,对苦豆子种子和幼苗进行胁迫处理,测定了不同处理条件下种子的最终发芽率、发芽势(第5天)和相对盐害率、旱害率及幼苗的相对电导率、叶绿素含量。结果表明苦豆子种子和幼苗能耐受的NaCl溶液浓度的临界值分别为0.3 mol/L和0.4 mol/L,能耐受的PEG-6000质量分数的临界值分别为15%和20%,表明苦豆子是一种具有极强耐盐、耐旱性的植物。该结果为苦豆子在荒漠区生态防护林的选材提供了依据,也有助于苦豆子耐盐、耐旱功能基因的发掘。

Sophora alopecuroides is used windbreak and sand-fixation plants because of its characteristics for resistance to salt and drought in the desert area. In this paper, the seed and seeding were treated by 0.2 mol/L, 0.3 mol/L,0.4 mol/L, 0.5 mol/L NaCl solution, 5%, 10%, 15%, 20%, 25% PEG-6000 solution, respectively. And then we measured the germination rate, germinability, relative salt harm rate and drought harm rate of seed. Meanwhile the relative electrolytic leakage and chlorophyll content of seeding leaves were measured under salt and drought treatment. The experimental results showed that the Sophora alopecuroides seeds and seedlings could tolerate to NaCl solution were 0.3 mol/L and 0.4 mol/L, and PEG-6000 were 15% and 20% respectively. All showed that Sophora alopecuroides was a kind of characteristics for resistance to salt and drought plant. The results of Sophora alopecuroides could provide basis of desert ecological shelter forest in the selection, and also be helpful for salt tolerance,drought-tolerant gene discovery.