乙烯刺激对橡胶树磷素营养代谢的影响

Effect on Phosphorus Metabolism Disorders by Ethylene Stimulus on Heavea brasiliensis

为分析乙烯对橡胶树磷素营养代谢的影响,以PR107、RY7-33-97、RY8-79三个品系为材料进行总固形物含量、全磷、无机总磷、B-乳清无机磷、C-乳清无机磷、核酸等生理指标与磷转运蛋白Hbpht1:1基因表达在一个乙烯刺激周期内相关性分析。结果表明:不同品系的橡胶树对乙烯敏感性不同:PR107对乙烯刺激最敏感、RY7-33-97次之、RY8-79最不敏感。每个刀次内,乙烯刺激割胶使Pi随排胶时间延长不断流失,乙烯处理组与对照组磷转运基因Hbpht1:1表达量均有不同程度上升,其中,乙烯处理组表达量显著高于乙烯不处理组。磷转运基因Hbpht1:1表达量变化与生理指标的变化有明显相关性。本研究为橡胶分子育种提供依据。

The objection of this study is to analyze the effect on phosphorus metabolism disorders by ethylene stimulus on Heavea brasiliensis, taking rubber latex of＂PR107＂,＂RY7-33-97＂,＂RY87-9＂as test materials. The discrepancy analysis on latex physiology such as total solids content, total phosphorus content, total inorganic phosphorus, inorganic phosphorus of latex B-serum and latex C-serum content, nucleic acids content and the gene expression of Pi transporter Hbpht1： 1. Results showed that different strains of rubber tree have different reaction to the ethrel stimulation： the most sensitive to the ethrel stimulation was PR107, the second was RY7-33-97, the least was RY8-79. Combined with the physiological index related to phosphorus and the Hbpht1： 1 expression changes, there was a correlation between Hbpht1： 1 expression and adversity physiological indexes. And the Hbpht1：1 expression in stimulated rubber tree by ethylene was higher than that of not stimulated. So that, the function of Hbpht1： 1 may be relevant to the latex physiology. This study provides a basis for further research on molecular breeding of rubber.