鞣花酸预防高脂饮食诱导的大鼠肥胖的作用研究

Anti-obesity Function of Ellagic Acid on High-fat Diet-induced Rats

目的:研究鞣花酸对高脂饮食引起的大鼠肥胖预防的影响。方法:将40只SD雄性大鼠按体重随机分为鞣花酸高剂量组(高脂饮食+30 mg/kg/d鞣花酸,EAH),鞣花酸低剂量组(高脂饮食+10 mg/kg/d鞣花酸,EAL),阳性对照组(高脂饮食+37.8mg/kg/d奥利司他,O),模型对照组(高脂饮食+10 mL/kg/d 0.5%CMC钠溶液,HFD)和空白对照组(普通饮食+10 mL/kg/d 0.5%CMC钠溶液,ND),连续给药10周,测量体重、进食量、体脂、肝重、肾重,计算脏器系数和脂肪系数,测定血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C);HE染色观察脂肪细胞和肝脏组织细胞形态。结果:干预10周后,与模型对照组比较,鞣花酸高、低剂量组大鼠体重和进食量均显著下降(P<0.05),脂肪系数显著降低(P<0.001);鞣花酸高、低剂量组血清HDL-C增加(P<0.01),鞣花酸高剂量组血清TC降低(P<0.05);HE染色观察发现鞣花酸高、低剂量组脂肪细胞直径显著变小,肝组织脂滴空泡明显减少,且呈剂量依赖性。结论:鞣花酸可能通过抑制高脂饮食大鼠的摄食量从而抑制其体重的增加。

Objective： To investigate the effect of Ellagic Acid on obesity prevention. Methods： Forty male SD rats were randomly divided into 5 groups （ n = 8 ） as follows： rats fed with HF diet with high dosage of Ellagic Acid（ 30 mg/kg/d, EAH group）, rats fed with HF diet with low dosage of Ellagic Acid （ 10 mg/kg/d, EAL group）, rats fed with HF diet with Orlistat Capsules（ 37.8 mg/kg/d, O group）, rats fed with HF diet with placebo pellet （0.5 % CMC Na solution, 10 mL/kg/d, HFD group）, and rats fed with normal diet with placebo pellet （0.5 % CMC Na solution, 10 mL/kg/d, ND group） for 10 weeks. Body weight, food intake, the index of body adipose tissue, liver and kidney were measured. The serum lipid level of TG, TC, HDL-C and LDL-C were analyzed. Adipocyte and liver cells were examined under a light microscope after HE staining. Results： Ellagic Acid high and low dosage could significantly decrease body weight, food intake and body adipose tissue index compared with HFD group （P 〈 0.05）. All dosage of Ellagic Acid could significantly increase Serum HDL-C（P 〈 0.01 or P 〈 0.05 ）, and the high dose Ellagic Acid could significantly decrease serum TC（P 〈 0.05 ）. The size of lipocytes of adipose tissue and the number of lipid droplet of liver of the high and low dosage of Ellagic Acid became smaller and littler. Conclusion： Ellagic acid could significantly inhibit body weight by inhibiting food intake in rats fed with high fat diet.