呼吸窘迫综合症患儿外周血单个核细胞中CD24和炎症因子mRNA的表达水平及其预后价值

mRNA Expression of CD24 and Inflammatory Factors in Peripheral Blood Mononuclear Cell of Children with Respiratory Distress Syndrome and Its Prognostic Value

目的:通过检查呼吸窘迫综合症患儿外周血单个核细胞中CD24和TNF-α、IL-6、和IL-17A炎症因子mRNA的表达,探讨其对呼吸窘迫综合症的诊断和预后价值。方法:选择2015年1月至12月在我院接受治疗的32例非感染型呼吸窘迫综合症患儿为研究组,选择同期的30例健康新生患儿为对照组,采集研究组治疗前后和对照组的外周血,分离单个核细胞,采用RT-PCR检测CD24和TNF-α、IL-6、和IL-17A炎症因子mRNA的表达水平。结果:研究组治疗前CD24mRNA表达水平明显高于对照组,差异有统计学意义(P<0.01),而TNF-α、IL-6、和IL-17A mRNA表达水平比较,差异无统计学意义(P>0.05)。研究组治疗后CD24mRNA表达水平明显低于治疗前,差异有统计学意义(P<0.01),而TNF-α、IL-6、和IL-17A mRNA表达水平比较,差异无统计学意义(P>0.05)。结论:呼吸窘迫综合症患儿外周血单个核细胞中CD24mRNA表达水平升高,可能是其诊断和预后的分子标记物。

Objective： Through detecting the mRNA expression of CD24 and TNF-α, IL-6, IL-17A in peripheral blood mononuclear cell of children with respiratory distress syndrome, to explore its diagnosis and prognostic value for respiratory distress syndrome. Methods： Selected 32 children with non-infectious respiratory distress syndrome as the study group who treated in our hospital from January to December 2015,and selected 30 health newborns as the control group in the same period, then collected the peripheral blood in study group before and after treatment and in control group respectively, isolated mononuclear cells, and detected the mRNA expression levels of CD24 and TNF-α, IL-6, IL-17A by RT-PCR method. Results： mRNA expression level of CD24 in study group before treatment was higher than control group, the difference was statistically significant （P〈0.01）, but there was no statistical difference on mRNA expression levels of TNF-α, IL-6, IL-17A in two groups （P〉0.05）. mRNA expression level of CD24 in study group after treatment was lower than before treatment （P〈0.01）, but there was no statistical difference on mRNA expression levels of TNF-α, IL-6, IL-17A （P〉0.05）. Conclusion： mRNA expression level of CD24 is increasing in peripheral blood mononuclear cell of children with respiratory distress syndrome, which may be the molecular markers for its diagnosis and prognosis.