模拟海拔4500米高原环境致大鼠肾脏适应性损伤的评估

Evaluation of the Simulating Plateau Environment of 4500 Meters Altitude induced Renal Adaptability Impairment in Rat

目的:探讨模拟海拔4500米高原环境致大鼠肾适应性损伤的情况。方法:通过检测模拟海拔4500米高原环境下大鼠血清肌酐(Scr)、中性粒细胞明胶酶相关载脂蛋白(NGAL)及尿蛋白,选择成年雄性SD大鼠130只,实验组80只大鼠通过低压氧舱模拟海拔4500米高原缺氧环境,对照组50只给予常规饲养,于0、14、28 d留取大鼠血液、尿液,测定Scr、NGAL及尿蛋白水平。结果:实验组14、28 d Scr、NGAL及尿蛋白水平较对照组明显升高,差异具有统计学意义(P<0.05),实验组内28 d Scr、NGAL及尿蛋白水平均14 d下降趋势明显,差异均具有统计学意义(P<0.05)。结论:急性缺氧早期大鼠肾发生一定适应性损伤,后期有所降低。

Objective： To evaluate the renal adaptability impairment under the simulating plateau environment of 4500 meters alti- tude in rat. Methods： The levels ofcreatinine and neutrophil gelatinase associated lipocalin （NGAL） in the serum and urine protein of rat under the simulating plateau environment of 4500 meters altitude were detected. 130 male SD rats were chosen, including 80 rats as observation group （breeded under low pressure oxygen tank to duplicate the model of the simulating the plateau environment of 4500 meters altitude） and 50 rats as control group （breeded under common conditions）. Results： The levels of creatinine, NGAL, and urine protein of control group at 14 and 28 d were lower than those at 14 d in the observation group （P〈0.05）. The levels of Scr, NGAL and urine protein levels of rat at 28 d were lower than those at 14 d in the observation group. Conclusions： At early stage of acute hypoxia, renal adaptability impairment could be observed under the plateau hypoxia environment, which could be eased at later stage.