氟对人成骨细胞和小鼠骨组织caspase14表达影响

Effect of Fluoride on the Expression of Caspase14 in Human Osteoblasts and Bone Tissue in Mice

目的:初步探讨氟对人成骨细胞saos-2和小鼠骨组织中caspase14表达的影响。方法:体外建立人成骨细胞染氟模型,以不加入氟入培养基者为空白对照,实验组培养基内分别含氟化钠5、10、20、40 mg/L。以氟化钠加入饮水中,建立小鼠染氟模型。应用Western blot法检测不同剂量氟(0、5、10、20、40 mg/L)对成骨细胞saos-2中caspase14的表达,再用免疫组织化学检测不同染氟剂量(0、50、100、150 mg/L)染氟小鼠骨骼上caspase14的表达情况。结果:Caspase14在成骨细胞saos-2中各实验组表达明显上调(均P<0.01),5 mg/L组表达最高。染氟小鼠模型骨组织中亦有表达,染氟组表达均高于对照组(均P<0.05),且有逐渐增高趋势。结论:Caspase14在染氟成骨细胞和骨组织中的高表达参与了氟诱导细胞凋亡的过程。

Objective： To study the effect of fluoride on the expression of caspasel4 in human osteoblasts Saos-2 cells and bone tissues in mice. Methods： The model was established using human osteoblasts exposed to fluoride in vitro, by adding fluorine into the culture medium as blank control, and the culture medium of the experimental group contained sodium fluoride respectively 5, 10, 20 and 40 mg/L. By adding sodium fluoride into drinking water, the fluoride model of mice was established. Using Western blot method, the effect of different doses of fluoride （0, 5, 10, 20, 40 mg/L） on the expression of caspasel4 in human osteoblasts Saos-2 cells was detected. Then the immunohistochemistry was used to detect the effect of different doses of fluoride （0, 50, 100 and 150 mg/L） on the caspasel4 expression in mouse skeletal tissues. Results： The expression of caspasel4 in the experimental group of Saos-2 cells was up-regulated（all P〈0.01 ）, and it was highest in the 5 mg/L group. Caspasel4 were also expressed in bone tissues of fluorosis-treated mice model, and the expression of caspase14 in fluoride group of different dose was higher than that of control group with a gradual increase trend （all P 〈 0.05）. Conclusions： The high expression of caspasel4 in osteoblasts and bone tissues is involved in the process of apoptosis induced by fluoride.