长期低浓度七氟醚吸入对雄性小鼠生殖功能的影响

Effects of Long-Term Low-Dose Sevoflurane Exposure on Reproduction of Male Mice

目的:探讨长期低浓度七氟醚吸入对雄性小鼠生殖功能的影响。方法:雄性C57小鼠20只随机分为吸醚组和对照组,吸醚组给予连续45 d,每天4 h吸入0.2%七氟醚。吸醚第36 d起每只雄性小鼠与4只雌性小鼠合笼交配。合笼结束后,雄性小鼠称重,计算睾丸指数,观察睾丸形态学变化,检测附睾精子活力和血清激素水平,统计子代小鼠数量和性别比例。结果:长期低浓度七氟醚吸入后雄性小鼠体重增长明显减少,精子活力显著降低,血清卵泡刺激素(FSH),促黄体生成素(LH),瘦素(LEP)显著升高,雄激素(T)水平无显著差异。雌性小鼠受孕率、子代数量及性别比例无显著差异。结论:长期低浓度七氟醚吸入对雄性小鼠具有一定的生殖毒性,影响其血清激素水平和精子活力,但受孕率和子代性别比无显著差异。

Objective： To investigate the effects of long-term low-dose sevofiurane inhalation on reproductive function of male mice. Methods： Twenty male C57 mice were randomly divided into two groups （n=10）： control group and Sevo group. The mice in Sevo group exposed to 0.2% sevoflurane for 45 d, 4 h/day. Thirty five days later, each male mouse mated with 4 female mice. Ten days later, these male mice were weighed and testis index was calculated. The morphological changes of the testes, the sperm motility and serum hormone levels were detected. The number of offspring mice and sex ratio were calculated. Results： After Long-term low-dose sevof/urane inhalation, the weight increasement of male mice and sperm motility significantly decreased, and the serum levels of FSH, LH, LEP significantly increased, whereas the serum levels of Teststerone did not change compared with mice in Con group. And there were no significant difference in pregnancy rate, offspring number and sex ratio between Con group and Sevo group. Conelusions： Long-term low-dose sevoflurane exposure affected serum hormone level and significantly decreased sperm motility of the male mice, but had no influence on pregnancy rate and the number and sex ratio of offsprings. These findings indicate that long-term low-dose sevoflurane inhalation exerts toxicity on reproduction of male mice.