假胶素A在天然蜂胶中的不存在性研究

A Research on the Inexistence of Fraudpropolin A in Natural Propolis

本研究旨在验证此前建立的蜂胶掺伪检测专利技术的可靠性。方法:参照专利方法,采用Kromasil100-5C18柱(250 mm×4.6 mm,5μm),以甲醇-0.5%磷酸水为流动相进行梯度洗脱,体积流量1 m L·min-1,检测波长296 nm,假胶素A为对照品,HPLC检测已知来源的纯天然蜂胶样品[1]。结果:134种不同类型和不同产地的纯天然蜂胶中,均未检出假胶素A。结论:验证了专利方法的可靠性,证明该方法可用于蜂胶掺伪检测。

This study aimed at verifying a previous patented fraud detection method of propolis. In accordance with thepatented process, the chromatographic separation was achieved on a Kromasil 100-5C18 column（250 mm × 4.6 mm, 5 μm） asmobile phrase A was methanol and mobile phrase B was water（0.5% phosphoric acid） at the flow rate of 1 m L·min-1forgradient elution. The detection wave length was 296 nm. Fraudpropolin A was taken as the reference, while the knownsources of natural propolis were determined by HPLC. As a result, no fraudpropolin A was detected in the 134 sources ofnatural propolis at different types or from various origins. It was concluded that the patented process was sound in thefraud detection method of propolis.