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假胶素A在天然蜂胶中的不存在性研究 被引量:1

A Research on the Inexistence of Fraudpropolin A in Natural Propolis
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摘要 本研究旨在验证此前建立的蜂胶掺伪检测专利技术的可靠性。方法:参照专利方法,采用Kromasil100-5C18柱(250 mm×4.6 mm,5μm),以甲醇-0.5%磷酸水为流动相进行梯度洗脱,体积流量1 m L·min-1,检测波长296 nm,假胶素A为对照品,HPLC检测已知来源的纯天然蜂胶样品[1]。结果:134种不同类型和不同产地的纯天然蜂胶中,均未检出假胶素A。结论:验证了专利方法的可靠性,证明该方法可用于蜂胶掺伪检测。 This study aimed at verifying a previous patented fraud detection method of propolis. In accordance with thepatented process, the chromatographic separation was achieved on a Kromasil 100-5C18 column(250 mm × 4.6 mm, 5 μm) asmobile phrase A was methanol and mobile phrase B was water(0.5% phosphoric acid) at the flow rate of 1 m L·min-1forgradient elution. The detection wave length was 296 nm. Fraudpropolin A was taken as the reference, while the knownsources of natural propolis were determined by HPLC. As a result, no fraudpropolin A was detected in the 134 sources ofnatural propolis at different types or from various origins. It was concluded that the patented process was sound in thefraud detection method of propolis.
出处 《世界科学技术-中医药现代化》 CSCD 2017年第2期300-305,共6页 Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
关键词 蜂胶 假蜂胶 杨树胶 假胶素A Propolis fraud propolis poplar tree gum fraudpropolin A
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