微小RNA-182在系统性红斑狼疮中的诊断价值

Value of microRNA-182 in diagnosis and prediction for systemic lupus erythematosus

目的:观察microRNA-182在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血的表达水平,明确microRNA-182应用于SLE诊断的临床价值。方法:选取2014年1月至2015年1月在南京医科大学附属无锡市第二人民医院就诊的SLE患者80例(SLE组),同期招募健康体检者70例(对照组)。2组患者入院即刻抽取约5 mL静脉血,采用Taq Man real-time PCR方法测定所有受试者外周血microRNA-182表达水平。根据SLE患者是否首次发病,分为初发SLE亚组及非初发SLE亚组,并比较两亚组患者microRNA-182表达水平的差异。分析microRNA-182水平与抗ds-DNA抗体及SLE疾病活动度指数(systemic lupus erythematosus disease activity index,SLEDAI)的相关性。结果:SLE患者外周血中microRNA-182水平较健康对照组显著升高(1.58±0.69 vs.0.40±0.35,P<0.01),其中初发SLE患者microRNA-182水平较非初发SLE患者显著升高(2.05±0.56 vs.1.10±0.45,P<0.01),同时发现microRNA-182水平与SLEDAI(r=0.466,P<0.01)及抗ds-DNA抗体(r=0.442,P<0.01)具有显著相关性。同时,ROC曲线发现microRNA-182在诊断SLE方面具有较高价值[AUC:0.94(95%CI0.91~0.98)]。结论:microRNA-182可以作为诊断及SLE的生物标志物。

Objective：To study the expression of microRNA-182 in systemic lupus erythematosus（SLE） for exploring the value of diagnosis for SLE.Methods：Eighty SLE patients served as a SLE group and 70 healthy volunteers as a healthy control（HC） group from Wuxi Second People＇s Hospital of Nanjing Medical University between January 2014 and January 2015.About 5 mL venous blood was collected from all subjects on admission,and the levels of microRNA-182 were detected by Taq Man real-time（RT） PCR.Meanwhile,SLE patients were divided into a newly diagnosed subgroup（n=40） and a non-newly diagnosed subgroup（n=40）,the difference of expression microRNA-182 were compared in the two subgroups.The relationship between microRNA-182 levels and anti-ds-DNA antibody and SLEDAI were explored subsequently.Results：The levels of microRNA-182 in the SLE group were significantly higher than the HC group（1.58±0.69 vs.0.40±0.35,P〈0.01）,meanwhile its levels in newly diagnosed subgroup were significantly higher than non-newly diagnosed subgroup（2.05±0.56 vs.1.10±0.45,P〈0.01）.The expression of microRNA-182 had a positive correlation with anti-ds-DNA antibody and SLEDAI（r=0.466,P〈0.01 and r=0.442,P〈0.01,respectively）,and ROC suggested that the value of microRNA-182 in diagnosis for SLE was high [AUC：0.94（95% CI 0.91–0.98）].Conclusion：microRNA-182 is a potential biomarker in diagnosis for SLE.