血性胸腹水涂片中红细胞去除方法的探讨

Study on the removal method of red blood cells in bloody pleural effusion smear

目的探讨去除血性胸腹水涂片中红细胞的方法,为临床提高病理学细胞诊断率提供参考。方法收集清远市人民医院临床科室送检的新鲜血性胸腹水标本32例为研究样本。每例分为对照组与实验组,实验组加入0.5%苦味酸,与不做任何处理并按照传统方法涂片的对照组进行比较。结果对照组背景见大量红细胞;实验组红细胞处理完全,背景清晰,且不影响其他细胞形态。结论 0.5%苦味酸能去除血性胸腹水涂片背景中的红细胞,利于血性胸腹水细胞学的诊断,在临床诊断中值得推广及应用。

Objective To explore the removal method of red blood cells in bloody pleural effusion smear, provide reference for clinical improve diagnostic pathology cell. Methods 32 samples of fresh bloody pleural effusion were collected as samples from clinical departments in Qingyuan People＇s Hospital. Divided into control group and experimental group, adding 0.5% picric acid in the experimental group, compared with the control group with conventional treatment.Results A large number of red blood cells were found in the control group.The experimental group had a clear background without any red blood ceils, and did not affect the morphology of other cells. Conclusions 0.5% picric acid can remove the red blood cells in the hemorrhagic chest water smear, is helpful to diagnosis of bloody pleural effusion cytology, worthy of popularization and application in clinical diagnosis.