胶膜菌属真菌与兰科植物的共生体系建立

Symbiosis Established Between Orchid and Tulasnella spp. Fungi

为建立兰科植物与菌根真菌的共生体系,本研究筛选出适宜的共生培养基,采用ITS-rDNA分析鉴定了6株菌根真菌,并将6株菌与兰科植物共生培养,利用台盼蓝染色法检验共生体是否建立。结果表明,2.5 g·L^(-1)燕麦琼脂培养基为树兰和胶膜菌属真菌共生的最适宜培养基,供试兰科植物中仅树兰可与6株胶膜菌属真菌共生,其中一株美孢胶膜菌(Tco 2)促进树兰种子萌发和原球茎发育的效果最好。本研究筛选出可促进树兰高效萌发成苗的共生真菌和培养基,为真菌与植物共生关系研究提供了良好的试验系统,为树兰的生产繁殖奠定了基础,对保护兰科植物物种资源具有重要意义。

In this study, the ITS-rDNA sequence analysis was used to identify six strains of mycorrhizal fungi, then co- culture them with orchids, and the trypan blue staining test was used to verify the symbiosis, in order to establish the symbiosis between orchid and mycorrhiza, and also to screen a suitable medium for symbiotic culturing. The results showed that all Tulasnella spp. strains in this study can establish symbiosis with Epidendrum secundum, and 2.5 g·L^-1 oatmeal agar medium was the most suitable co-culture medium for them, a strain effectively promoted the seed germination and protocorm differentiation of Epidendrum secundum was Tulasnella calospora （Tco2）. The mycorrhizal fungi and co-culture medium screened for promoting the seed germination of Epidendrum secundum provide the fungi and plant symbiotic relationship research a good experimental system model, which will play a foundation for reproducing and it would be of great significance on protection of species resource of Epidendrum secundurn.