乌贼墨多糖提取物的制备及其对B16F10细胞的影响

Preparation of Polysaccharide Extracts From Sepia Ink and Its Effects on B16F10 Cells

为了探索乌贼墨多糖(SIP)对体外培养的B16F10细胞的生物学效应,以乌贼墨为原材料,对SIP进行提取分离,并研究SIP提取物对B16F10细胞活力、细胞内酪氨酸酶活性及黑色素合成等的影响。结果表明,SIP提取的最优条件为提取温度40℃,料液比1∶3,提取时间4h,提取次数3次,在此条件下SIP得率为14.12 mg·g^(-1)。SIP提取物对B16F10细胞形态有一定影响,对细胞活性、酪氨酸酶活性和黑色素合成均有一定抑制作用,且呈现一定剂量效应。综上可知,SIP提取物能抑制黑色素合成,具有作为美白剂的发展前景,这为乌贼墨合理利用提供了一定科学依据。

In order to reveal the bio-efficacy of Sepia ink polysaccharides （SIP） on B16F10 cell lines in vitro, the separation of SIP from Sepia ink, and the effects of SIP extracts on the proliferation rate of cells, tyrosinase activity and melanin synthesis were investigated. The results showed that the optimum process for SIP extraction was obtained with the material-liquid ratio of 1∶3 at 40℃ for 4 hours thrice. Under the optimum conditions, the yield of SIP was 14.21mg·g^-1. The cell morphology of the cells changed due to the treatment of SIP extracts. The proliferation rate of B16F10 cells, the tyrosinase activity and the synthesis of melanin were all suppressed or down-regulated by SIP extracts, and this inhibitory effect had direct correlation with the concentration of SIP extracts. The results suggested that SIP extracts could inhibit the synthesis of melanin, which resulted to white skin. This research provides a scientific basis for the use of sepia.